GromSil 300 HIC, 7 μm, µm (250 × 4.6 mm)
Hydrophobic Interaction Chromatography of Proteins
1
Cytochrome C
2
Myoglobin
3
β-Lactoglobulin
4
Ribonuclease A
5
Lysozyme
6
α-Chymotrypsin
7
Chymotrypsinogen
| Substances |
Cytochrome C
Myoglobin β-Lactoglobulin Ribonuclease A Lysozyme α-Chymotrypsin Chymotrypsinogen |
|---|---|
| Eluent | A: 2.0 M (NH₄)₂SO₄ - 0.1 M KH₂PO₄, pH 6.8 B: 0.1 M KH₂PO₄, pH 6.8 |
| Flow Rate | 1.0 ml / min |
| Detection | 280 nm |