GromSil FMOC 1, µm (250 × 4.6 mm)
Amino Acid Analysis employing Precolumn Derivatisation with FMOC followed by Pentane Extraction
1
Cys-SO₃H
2
Asp
3
Ser
4
Glu
5
Thr
6
Gly
7
Arg
8
Ala
9
Tyr
10
Pro
11
Met
12
FMOC-OH
13
Val
14
Nor-Val
15
Phe
16
Ile
17
Leu
18
His
19
Lys
| Substances |
Cys-SO₃H
Asp Ser Glu Thr Gly Arg Ala Tyr Pro Met FMOC-OH Val Nor-Val Phe Ile Leu His Lys |
|---|---|
| Eluent | A: 50 mM Na-acetate, pH 4.2 / ACN = 80 / 20 B: 50 mM Na-acetate, pH 4.2 / ACN = 20 / 80 |
| Flow Rate | 1 ml / min |
| Detection | 263 nm exc., 313 nm em |