ReproSil OPA, 3 µm (150 × 4.6 mm)
Analysis of Amino Acids by pre-column Derivatisation with OPA/3-MPA
1
1. Asparaginic acid
2
2. Glutamic acid
3
3. Asparagine
4
4. Serine
5
5. Glutamine
6
6. Glycine
7
7. Threonine
8
8. Histidine
9
9. Citrulline
10
10. Alanine
11
11. Taurine
12
12. Arginine
13
13. a-amino butyric acid
14
14. Tyrosine
15
15. Valine
16
16. Methionine
17
17. N-Valine
18
18. Tryptophane
19
19. Isoleucine
20
20. Phenylalanine
21
21. Leucine
22
22. Ornithine
23
23. Lysine
| Substances |
1. Asparaginic acid
2. Glutamic acid 3. Asparagine 4. Serine 5. Glutamine 6. Glycine 7. Threonine 8. Histidine 9. Citrulline 10. Alanine 11. Taurine 12. Arginine 13. a-amino butyric acid 14. Tyrosine 15. Valine 16. Methionine 17. N-Valine 18. Tryptophane 19. Isoleucine 20. Phenylalanine 21. Leucine 22. Ornithine 23. Lysine |
|---|---|
| Eluent | 60 µm |
| Flow Rate | 1 ml / min |
| Detection | Fluor: Ex 330nm EM. 450 nm |
| Eluent A | 25mM Na-phosphate-buffer (pH=7,2)/THF (995/5) |
| Eluent B | 25mM Na-phosphate-buffer (pH=7,2) MeOH/ACN (50/35/15) |
| Gradient | 0-3 min. 0% B, beià at 20 min 35% B, 25 min 50%, 36 min 60 B, 40 min 70%, 43-45 min 100% B |
| Product number | R13.opa.s1546 |