Exsil Pure 120 SAX, 3 µm (250 × 4.6 mm)

Isocratic HPLC Separation of Six Water Soluble Vitamins & Their Phosphates

1 Riboflavin (B2)
2 Pyridoxine (B6)
3 Ascorbic Acid (C)
4 Folic Acid (B9)
5 B2-phosphate #1
6 B2-phosphate #2
7 B2-phosphate #3
8 B6-phosphate
Graph of Isocratic HPLC Separation of Six Water Soluble Vitamins & Their Phosphates with Exsil Pure 120 SAX, 3 µm (250 x 4.6 mm)
Substances Riboflavin (B2)
Pyridoxine (B6)
Ascorbic Acid (C)
Folic Acid (B9)
B2-phosphate #1
B2-phosphate #2
B2-phosphate #3
B6-phosphate
Eluent AcN-(50mM NH₄HPO₄+0.05%H₃PO₄) 65:35
Flow Rate 1.5 mL / min
Detection 260 nm and 300 nm

Application for Column:

Exsil Pure 120 SAX Particle Size: 3 µm
Column Size: 250 × 4.6 mm
Pore Size: 120 Å

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