GromSil 300 is a group of HPLC columns from Dr. Maisch HPLC GmbH, based on fully porous, high-purity silica with a 300 Å pore size. This platform is specifically engineered for the separation of large biomolecules such as intact proteins, monoclonal antibodies, peptides, and glycoproteins. The wide-pore architecture allows unhindered analyte access to the stationary phase, ensuring effective mass transfer and reproducible retention for high-molecular-weight compounds.
The group includes multiple bonded chemistries – such as C18, C8, and Phenyl – and supports reversed-phase LC-MS and UV detection workflows under acidic to neutral conditions.
The GromSil 300 group from Dr. Maisch HPLC GmbH delivers reliable high-resolution performance for demanding bioanalytical separations — ideal for laboratories working with large, complex analytes.
| Particle Sizes | 3 – 10 µm |
|---|---|
| Phases/Modifications | C4, C18, CN, Diol, NH2, Si, TMS (C1) |
| Pore Sizes | 300 Å |
Fully porous, high-purity silica with 300 Å pore size
Bonded phases include C18, C8, and Phenyl for versatile selectivity
Designed for large biomolecules and complex protein structures
Excellent peak symmetry, efficiency, and mechanical stability
Available in 3 µm, 5 µm, and 10 µm particle sizes
Intact proteins, antibody fragments, and therapeutic peptides
Peptide mapping and bioanalytical LC-MS workflows
Reversed-phase separations under low-pH mobile phases
Method development for large or tailing-prone biomolecules
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| GromSil 300 Amino-4 PR | 3 – 7 µm | 300 Å | |
| GromSil 300 Butyl-1 ST | 3 – 10 µm | 300 Å | |
| GromSil 300 Butyl-2 FE | 3 – 5 µm | 300 Å | |
| GromSil 300 Cyan-1 ST | 3 – 10 µm | 300 Å | |
| GromSil 300 Diol | 3 – 5 µm | 300 Å | |
| GromSil 300 NP-1 | 5 µm | 300 Å | |
| GromSil 300 ODS-5 ST | 3 – 10 µm | 300 Å | |
| GromSil 300 SEC | 5 µm | 300 Å | |
| GromSil 300 TMS-1 ST | 3 – 5 µm | 300 Å |