The Prosphere 300 series is a family of wide-pore (300 Å) silica-based HPLC columns specifically designed for the separation of biomolecules such as peptides, proteins, and oligonucleotides. The large pore structure provides excellent accessibility for high-molecular-weight analytes, ensuring sharp peak shapes and reliable performance.
Available with different bonded chemistries – including C18, C8, and specialty phases – Prosphere 300 columns offer tailored selectivity for a wide range of pharmaceutical, biopharmaceutical, and biochemical applications. The robust bonding technology ensures reproducibility, stability, and long operational lifetimes, even under demanding chromatographic conditions.
The Prosphere 300 series delivers wide-pore performance with robustness and reproducibility – the reliable choice for laboratories analyzing peptides, proteins, and biomacromolecules.
| Particle Sizes | 3 µm, 5 µm, 10 µm |
|---|---|
| Phases/Modifications | C4, C8, C18, Phenyl |
| Pore Sizes | 300 Å |
| Surface Areas | 100 m²/g |
Wide-pore silica stationary phase (300 Å) for biomolecular separations
Available in multiple bonded chemistries
Optimized for peptides, proteins, and nucleic acids
High reproducibility and robust column lifetime
Sharp peak shapes and consistent performance
Pharmaceutical and biopharmaceutical analysis of therapeutic proteins and peptides
Proteomic studies requiring reliable wide-pore chromatography
Separation of oligonucleotides and other nucleic acids
Method development for biomolecular separations requiring tailored selectivity
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| Prosphere 300 C4 | 3 – 10 µm | 6 % | 300 Å |
| Prosphere 300 C8 | 3 – 10 µm | 8 % | 300 Å |
| Prosphere 300 C18 | 3 – 10 µm | 13 % | 300 Å |
| Prosphere 300 Phenyl | 3 – 10 µm | 300 Å |