The Mixed Mode column category from Dr. Maisch HPLC GmbH includes specialized HPLC phases that combine multiple retention mechanisms such as reversed-phase, ion exchange, and polar interactions. These columns are designed to handle challenging analytes that do not separate well using conventional single-mode HPLC, including zwitterions, charged drugs, strongly polar compounds, and complex mixtures with overlapping properties.
By offering orthogonal selectivity, Mixed Mode columns enable enhanced resolution, retention control, and method flexibility, particularly in pharmaceutical, bioanalytical, and chemical development workflows.
Mixed Mode columns from Dr. Maisch HPLC GmbH offer powerful retention flexibility and enhanced resolution – ideal for solving separation challenges where conventional HPLC phases fall short.
| Particle Sizes | 3 µm, 5 µm, 10 µm |
|---|---|
| Phases/Modifications | C8-NH2, C8-SAX, C8-SCX, C18-NH2, C18-COOH, C18-CN, C18-Phenyl |
| Pore Sizes | 100 Å, 120 Å |
| Surface Areas | 200 m²/g, 350 m²/g |
Dual or multi-mode retention (e.g. RP + ion exchange, RP + HILIC)
Suitable for zwitterionic, charged, and highly polar analytes
Supports gradient and isocratic elution with pH- and buffer-stability
Developed and quality-controlled by Dr. Maisch HPLC GmbH
Separation of ionizable pharmaceuticals and metabolites
Complex matrices requiring orthogonal selectivity
Mixed-mode method development and impurity profiling
HPLC of highly polar, acidic, or basic compounds
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| ReproSil Mixed-Mode Amino-C8 | 3 – 5 µm | 11 % | 100 Å |
| ReproSil Mixed-Mode Amino-C18 | 3 – 5 µm | 17 % | 100 Å |
| ReproSil Mixed-Mode C18-Acid | 5 – 10 µm | 20 % | 100 Å |
| ReproSil Mixed-Mode Cyano-C18 | 5 µm | 12 % | 100 Å |
| Reprospher 120 SAX-C8 | 10 µm | 120 Å | |
| Reprospher 120 SCX-C8 | 10 µm | 120 Å | |
| Reprospher Mixed-Mode Phenyl-C18 | 5 – 10 µm | 100 Å |