Prosphere is a column series specifically developed for the analysis and purification of peptides, small proteins, and other biomolecules using reversed-phase HPLC. Built on high-purity, fully porous silica with large pore sizes (typically 300 Å), Prosphere columns ensure optimal mass transfer, efficient interaction with large analytes, and excellent peak shape under gradient conditions.
With bonded phases such as C18, C4, and phenyl, the Prosphere series offers tailored selectivity for various peptide hydrophobicities and protein conformations, making it a reliable tool in biopharmaceutical, proteomic, and quality control workflows.
Prosphere columns provide reliable, high-efficiency peptide and protein separations – ideal for modern bioanalytical and biopharmaceutical HPLC workflows.
The product line was aquired by Dr. Maisch HPLC GmbH in 2016 from Grace / Alltech.
| Particle Sizes | 3 µm, 5 µm, 10 µm |
|---|---|
| Phases/Modifications | C4, C8, C18, C18-AQ, Phenyl |
| Pore Sizes | 300 Å |
| Surface Areas | 100 m²/g |
Wide-pore silica (e.g. 300 Å) ideal for peptides and proteins
Available with C18, C4, phenyl, and other reversed-phase chemistries
Strong mechanical stability and batch-to-batch consistency
Optimized for gradient elution with aqueous/organic systems
Suitable for analytical and semi-preparative scale
Peptide mapping and identification
Small protein and polypeptide separations
QC of therapeutic peptides and biosimilars
Gradient reversed-phase method development for biopharma
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| Prosphere 300 C4 | 3 – 10 µm | 6 % | 300 Å |
| Prosphere 300 C8 | 3 – 10 µm | 8 % | 300 Å |
| Prosphere 300 C18 | 3 – 10 µm | 13 % | 300 Å |
| Prosphere 300 Phenyl | 3 – 10 µm | 300 Å | |
| Prosphere HP 300 C4 | 3 – 10 µm | 5 % | 300 Å |
| Prosphere HP 300 C8 | 3 – 10 µm | 300 Å | |
| Prosphere HP 300 C18 | 3 – 10 µm | 13.5 % | 300 Å |
| Prosphere HP 300 C18-AQ | 3 – 10 µm | 14 % | 300 Å |