Macrosphere SEC phases are silica-based stationary phases developed for size exclusion chromatography (SEC) in both aqueous SEC and organic GPC systems. Designed for precise molecular size-based separations, these materials enable the characterization of compounds ranging from 250 Da to 1,250,000 Da, offering high resolution and reproducibility across a wide molecular weight range.
Built on high-purity spherical silica with carefully controlled pore structure, Macrosphere SEC phases ensure excellent diffusion and mass transfer properties for accurate molecular weight determination. Depending on the surface modification and mobile phase, the materials can be used in aqueous environments for the separation of proteins, peptides, and biopolymers, or in organic solvents for the analysis of synthetic polymers and oligomers.
The rigid silica support provides superior mechanical stability, long column lifetime, and consistent calibration behavior, making Macrosphere SEC an ideal choice for pharmaceutical, biochemical, and polymer research laboratories that demand precision and reproducibility.
Macrosphere SEC combines the precision of silica-based technology with versatile solvent compatibility – the reliable choice for accurate and reproducible size exclusion separations in both aqueous and organic chromatography systems.
| Particle Sizes | 1.9 µm, 3 µm, 5 µm |
|---|---|
| Phases/Modifications | Diol, Si |
| Pore Sizes | 60 – 800 Å |
Silica-based stationary phases for aqueous SEC and organic GPC
Broad molecular weight separation range from 250 Da to 1,250,000 Da
Controlled pore structure for accurate size-based separations
High-purity silica ensures mechanical robustness and reproducibility
Suitable for analytical and preparative chromatography
Molecular weight determination of proteins, peptides, and biopolymers (aqueous SEC)
Analysis of synthetic polymers and oligomers in organic GPC systems
Characterization of macromolecules across a broad size range
Method development and quality control in biochemical and polymer laboratories
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| Macrosphere 60 SEC | 5 µm | 60 Å | |
| Macrosphere 60 Si | 5 µm | 0 % | 60 Å |
| Macrosphere 100 SEC | 5 µm | 100 Å | |
| Macrosphere 100 Si | 3 – 5 µm | 0 % | 100 Å |
| Macrosphere 200 SEC | 1.9 – 5 µm | 200 Å | |
| Macrosphere 300 SEC | 5 µm | 300 Å | |
| Macrosphere 400 SEC | 5 µm | 400 Å | |
| Macrosphere 800 SEC | 5 µm | 800 Å |