The Prosphere HP 300 series comprises high-purity silica-based stationary phases with a 300 Å pore size, specifically engineered for the separation and analysis of proteins, peptides, nucleic acid fragments, and other biomolecules. The wide-pore structure ensures optimal accessibility and mass transfer for large molecules, enabling high efficiency and excellent peak symmetry.
Designed for biochemical and biopharmaceutical applications, Prosphere HP 300 columns deliver consistent performance, reproducibility, and durability under both gradient and isocratic conditions. Their high-purity silica support minimizes secondary interactions, ensuring accurate, reliable separations even for sensitive biomolecules.
The series includes a variety of bonded chemistries optimized for different analytical needs — from hydrophobic C18 and C8 phases for peptide mapping and protein analysis to polar and specialized surfaces for complex biopolymer separations.
Prosphere HP 300 combines high-purity silica technology with large-pore design – the reliable choice for precise and reproducible separations of biomolecules in protein, peptide, and nucleic acid analysis.
| Particle Sizes | 3 µm, 5 µm, 10 µm |
|---|---|
| Phases/Modifications | C4, C8, C18, C18-AQ |
| Pore Sizes | 300 Å |
| Surface Areas | 100 m²/g |
High-purity silica with 300 Å pore size for biomolecule separations
Optimized for proteins, peptides, and nucleic acid fragments
Excellent mass transfer and resolution for large molecules
High reproducibility, peak symmetry, and mechanical stability
Available with various bonded chemistries for flexible selectivity
Peptide mapping and protein analysis
Separation of nucleic acid fragments and biopolymers
Characterization of therapeutic proteins and enzymatic digests
Biopharmaceutical research and quality control
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| Prosphere HP 300 C4 | 3 – 10 µm | 5 % | 300 Å |
| Prosphere HP 300 C8 | 3 – 10 µm | 300 Å | |
| Prosphere HP 300 C18 | 3 – 10 µm | 13.5 % | 300 Å |
| Prosphere HP 300 C18-AQ | 3 – 10 µm | 14 % | 300 Å |