Organosilica hybrid particles are composite materials formed by co-condensation of inorganic silica precursors with organosilane compounds. The resulting hybrid matrix incorporates organic groups directly into the silica framework, creating a chemically reinforced structure that resists dissolution under both acidic and basic conditions.
Unlike conventional silica, which relies solely on Si–O–Si bonds, organosilica hybrids contain Si–C and Si–O–Si linkages, improving mechanical flexibility and surface stability.
These materials maintain the high surface area, controlled porosity, and uniform particle morphology typical of silica gels, while offering superior resistance to hydrolysis and phase degradation.
They serve as advanced supports for reversed phase, HILIC, ion exchange, and mixed-mode chromatography, ensuring reliable performance even under extended or harsh operating conditions.
Organosilica hybrid materials represent the next generation of stationary phases in liquid chromatography. By integrating organic components into the silica framework, they overcome the main limitations of pure silica — namely, restricted pH stability and reduced lifetime in aqueous basic environments.
These hybrid particles combine the best properties of both inorganic and organic materials: the mechanical rigidity and efficiency of silica with the chemical robustness and tunability of polymers.
As a result, they provide consistent retention behavior, longer column lifetime, and broader pH operating ranges (typically pH 1–10), making them ideal for modern high-performance separations in pharmaceutical, biotechnological, and environmental analysis.
Extended pH stability: Operable across a wide range (typically pH 1–10) without structural degradation.
Improved chemical resistance: Resistant to hydrolysis and dissolution under acidic or basic mobile phases.
High mechanical strength: Suitable for high-pressure HPLC and UHPLC operation.
Excellent reproducibility: Uniform surface chemistry and minimal silanol activity ensure consistent retention.
Low bleed and enhanced MS compatibility: Ideal for LC–MS and trace analysis applications.
Long column lifetime: Reduced surface deactivation and superior durability under repetitive gradients.
Organosilica hybrid particles are used in a broad spectrum of analytical and preparative chromatographic techniques, where both performance and chemical stability are critical. Typical applications include:
Reversed Phase Chromatography under aggressive gradient conditions and variable pH.
HILIC separations of polar and bioactive compounds with excellent reproducibility.
Ion Exchange and Mixed-Mode Chromatography, where hybrid surfaces minimize unwanted secondary interactions.
Pharmaceutical and biopharmaceutical analysis, including stability-indicating methods and impurity profiling.
LC–MS workflows, where low background and minimal stationary phase bleeding are required.
The combination of stability, selectivity, and versatility makes organosilica hybrid particles a leading choice for demanding chromatographic applications requiring both robustness and high efficiency.
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| ReproSil pHoenix C18 | 1.5 – 10 µm | 22 % | 100 Å |