Macrosphere 300 phases are silica-based ion exchange materials engineered for the separation and purification of biomolecules, including proteins, peptides, and nucleic acids. Built on high-purity spherical silica with a 300 Å pore size, these materials offer excellent accessibility for large biomolecules and enable efficient mass transfer, high loading capacity, and reproducible chromatographic performance.
The series is available in three ion exchange chemistries: SAX (Strong Anion Exchange) for negatively charged analytes such as nucleotides and acidic proteins, SCX (Strong Cation Exchange) for positively charged compounds including basic peptides and proteins, and WCX (Weak Cation Exchange) for more selective separations under milder buffer or pH conditions. This versatility allows Macrosphere 300 phases to deliver optimal selectivity and resolution across a wide range of biomolecular applications.
The rigid silica support ensures outstanding mechanical stability and reproducibility even under varying buffer compositions and gradient conditions, making these materials ideally suited for both analytical and preparative workflows in pharmaceutical, biotechnological, and biochemical laboratories.
Macrosphere 300 combines the precision of silica-based ion exchange with the accessibility of large-pore supports – the reliable choice for high-resolution and reproducible biomolecule separations.
| Particle Sizes | 5 µm, 7 µm |
|---|---|
| Phases/Modifications | SAX, SCX, WCX |
| Pore Sizes | 300 Å |
Silica-based stationary phases with 300 Å pore size for ion exchange chromatography
Available in SAX, SCX, and WCX chemistries for broad selectivity control
Optimized for separation and purification of biomolecules
High mechanical stability and reproducibility for long column lifetime
Suitable for analytical and preparative workflows
Ion exchange separation of proteins, peptides, and nucleic acids
Biopharmaceutical purification and quality control
Method development for biomolecule chromatography
Research and production in biotechnology and life sciences
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| Macrosphere 300 SAX | 5 µm | 300 Å | |
| Macrosphere 300 SCX | 5 µm | 300 Å | |
| Macrosphere 300 WCX | 7 µm | 300 Å |