ReproSil Mixed-Mode from Dr. Maisch HPLC GmbH is a versatile range of high-purity silica-based HPLC columns that combine two different retention mechanisms within a single stationary phase. By integrating reversed-phase hydrophobic bonding (such as C18 or C8) with polar or ion-exchange functionalities (such as amino, acidic, or cyano groups), these columns deliver orthogonal selectivity for complex mixtures containing analytes of varying polarity and charge.
This dual-mode capability allows analysts to fine-tune separations, making the ReproSil Mixed-Mode series ideal for method development and multi-class compound analysis in pharmaceutical, environmental, food, and specialty chemical applications.
ReproSil Mixed-Mode columns from Dr. Maisch HPLC GmbH provide versatile dual-mode separation power – the flexible choice for challenging and diverse HPLC applications.
| Particle Sizes | 3 µm, 5 µm, 10 µm |
|---|---|
| Phases/Modifications | C8-NH2, C18-NH2, C18-COOH, C18-CN |
| Pore Sizes | 100 Å |
| Surface Areas | 200 m²/g, 350 m²/g |
Fully porous, ultra-pure silica with mixed bonded phases
Combines hydrophobic reversed-phase and polar/ionic interactions
Broad selectivity range for diverse analytes
High efficiency, reproducibility, and peak symmetry
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Separation of mixtures with polar and non-polar compounds
Pharmaceutical API and impurity profiling
Natural product and metabolite analysis
Method development requiring orthogonal selectivity
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| ReproSil Mixed-Mode Amino-C8 | 3 – 5 µm | 11 % | 100 Å |
| ReproSil Mixed-Mode Amino-C18 | 3 – 5 µm | 17 % | 100 Å |
| ReproSil Mixed-Mode C18-Acid | 5 – 10 µm | 20 % | 100 Å |
| ReproSil Mixed-Mode Cyano-C18 | 5 µm | 12 % | 100 Å |