The ReproSil-Pur 1000 series is based on high-purity spherical silica particles with a 1000 Å pore size, developed for the separation of large biomolecules, peptides, proteins, and synthetic polymers. The very wide pore structure allows efficient diffusion and interaction of high-molecular-weight analytes, ensuring accurate separation and reproducible results.
ReproSil-Pur 1000 columns are available in three surface chemistries: C18, NH2, and unmodified silica (Si). The C18 phase provides strong hydrophobic retention and is particularly suitable for large hydrophobic biomolecules and peptides. The NH2 phase enables polar interactions and can be used for normal-phase or weak anion exchange chromatography. The unmodified silica variant is ideal for adsorption-based and normal-phase separations of macromolecules.
The ReproSil-Pur 1000 series combines high-purity silica with ultra-wide-pore accessibility – the dependable choice for laboratories analyzing very large biomolecules by HPLC.
| Particle Sizes | 3 µm, 5 µm, 10 µm |
|---|---|
| Phases/Modifications | C18, NH2, Si |
| Pore Sizes | 1 000 Å |
| Surface Areas | 25 m²/g |
Ultra-pure silica with 1000 Å pore size for very large biomolecules
Multiple bonded chemistries available (C18, NH2, and Si)
Optimized pore structure for proteins, glycoproteins, nucleic acids, and polysaccharides
Excellent reproducibility with sharp and symmetrical peaks
Robust bonding chemistry ensures long operational lifetimes
Biopharmaceutical analysis of intact proteins, antibodies, and glycoproteins
Nucleic acid and oligonucleotide separations under reversed-phase conditions
Polysaccharide and high-molecular-weight carbohydrate analysis
Proteomics workflows requiring ultra-wide-pore stationary phases
Method development for biomolecules beyond the range of 200–300 Å columns
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| ReproSil-Pur 1000 NH2 | 3 – 5 µm | 1 % | 1 000 Å |
| ReproSil-Pur 1000 ODS-3 | 5 – 10 µm | 1.6 % | 1 000 Å |
| ReproSil-Pur 1000 Si | 5 µm | 0 % | 1 000 Å |