The Reprospher 300 group from Dr. Maisch HPLC GmbH is based on fully porous, high-purity silica with a 300 Å pore size, developed specifically for the reversed-phase separation of large biomolecules. This platform includes a broad range of bonded phases such as C4, C8, C18, C30, Phenyl, and ion-exchange modifications tailored for proteins, glycopeptides, oligonucleotides, and complex therapeutic peptides.
Its large pore structure enables optimal mass transfer and accessibility for analytes with high molecular weight or complex secondary structures. These phases are compatible with acidic eluents and LC-MS workflows, delivering high efficiency, peak symmetry, and stability under demanding bioanalytical conditions.
The Reprospher 300 group from Dr. Maisch HPLC GmbH offers robust and versatile reversed-phase performance – ideal for high-resolution separations of biomolecules too large for conventional HPLC materials.
| Particle Sizes | 1.8 – 30 µm |
|---|---|
| Phases/Modifications | C4, C8, C18, PEI, Phenyl |
| Pore Sizes | 300 Å |
| Surface Areas | 100 m²/g |
Fully porous silica with 300 Å pore size for intact proteins and large peptides
Reversed-phase and ion-exchange chemistries: C18, C8, C4, C30, Phenyl, PEI
Optimized for TFA- and formic acid-based eluents
High mechanical and chemical stability across particle sizes
Available in 3 µm, 5 µm, and 10 µm configurations
Monoclonal antibodies, protein fragments, glycoproteins
Peptide mapping and LC-MS of large bioactive compounds
Therapeutic oligonucleotide separations
Bioanalytical and pharmaceutical method development
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| Reprospher 300 C4 | 1.8 – 5 µm | 2.5 % | 300 Å |
| Reprospher 300 C4-Aqua | 1.8 – 5 µm | 3 % | 300 Å |
| Reprospher 300 C4-DE | 2.5 – 5 µm | 3 % | 300 Å |
| Reprospher 300 C4-T | 5 µm | 4 % | 300 Å |
| Reprospher 300 C4-TN | 5 µm | 4 % | 300 Å |
| Reprospher 300 C8 | 1.8 – 5 µm | 4 % | 300 Å |
| Reprospher 300 C8-T | 5 µm | 6 % | 300 Å |
| Reprospher 300 C8-TN | 5 µm | 5 % | 300 Å |
| Reprospher 300 C18 | 1.8 – 10 µm | 7 % | 300 Å |
| Reprospher 300 C18-Aqua | 3 – 5 µm | 4 % | 300 Å |
| Reprospher 300 C18-DE | 2.5 – 5 µm | 7 % | 300 Å |
| Reprospher 300 C18-T | 5 µm | 11 % | 300 Å |
| Reprospher 300 C18-TN | 5 – 30 µm | 10 % | 300 Å |
| Reprospher 300 PEI | 3 – 10 µm | 4 % | 300 Å |
| Reprospher 300 Phenyl | 10 µm | 4 % | 300 Å |
| Reprospher 300 Phenyl-DE | 3 µm | 5 % | 300 Å |