The ReproSil-Pur 300 series is based on ultra-pure silica with a 300 Å pore size, specifically designed for the separation of proteins, large peptides, nucleic acids, and other high-molecular-weight biomolecules. The wide-pore structure ensures excellent accessibility for large analytes, maintaining high resolution and sharp peak shapes.
Available in a broad range of bonded chemistries (C18, C8, Phenyl, CN, etc.), the ReproSil-Pur 300 series provides tailored selectivity for reversed-phase, normal-phase, and aromatic separations. Robust bonding chemistry ensures reproducibility, durability, and long lifetimes even under demanding chromatographic conditions such as elevated temperatures and extended gradient runs.
The ReproSil-Pur 300 series combines high-purity silica with wide-pore design – the reliable choice for laboratories analyzing proteins and other large biomolecules by HPLC.
| Particle Sizes | 3 – 15 µm |
|---|---|
| Phases/Modifications | C4, C8, C18, CN, Diol, Phenyl, Si |
| Pore Sizes | 300 Å |
| Surface Areas | 100 m²/g |
High-purity silica with 300 Å pore size for large biomolecules
Wide range of bonded chemistries available (C18, C8, Phenyl, CN, etc.)
Enhanced accessibility and retention for proteins and peptides
Excellent reproducibility with sharp and symmetrical peak shapes
Robust bonding chemistry for long column lifetimes
Biopharmaceutical analysis of therapeutic proteins, antibodies, and peptides
Proteomics applications requiring wide-pore reversed-phase columns
Nucleic acid and oligonucleotide separations
Food and biochemical analysis of high-molecular-weight biomolecules
Method development for large analytes requiring wide-pore accessibility
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| ReproSil-Pur 300 C4 | 3 – 10 µm | 2.5 % | 300 Å |
| ReproSil-Pur 300 C8 | 3 – 15 µm | 4 % | 300 Å |
| ReproSil-Pur 300 CN | 3 µm | 300 Å | |
| ReproSil-Pur 300 Diol | 3 – 5 µm | 3.5 % | 300 Å |
| ReproSil-Pur 300 ODS-3 | 3 – 10 µm | 9 % | 300 Å |
| ReproSil-Pur 300 Phenyl | 3 – 5 µm | 3 % | 300 Å |
| ReproSil-Pur 300 Si | 3 – 10 µm | 0 % | 300 Å |