The Macrosphere series consists of high-purity silica-based stationary phases designed for size exclusion chromatography (SEC) and ion exchange chromatography (IEX). These materials combine the mechanical strength and efficiency of spherical silica with surface chemistries tailored for precise separations of biomolecules, synthetic polymers, and charged analytes.
In size exclusion chromatography, Macrosphere SEC phases offer highly controlled pore structures for accurate size-based separations across a wide molecular weight range. Their uniform silica matrix ensures excellent resolution, consistent calibration behavior, and reproducibility for both synthetic polymers and biological macromolecules.
For ion exchange chromatography, Macrosphere 300 phases are available with different ionic surface modifications, providing selective retention and separation of charged species such as proteins, peptides, and nucleic acids. The rigid silica backbone ensures mechanical stability, high loading capacity, and reliable performance under a wide range of pH and buffer conditions.
Macrosphere combines the precision of silica-based chromatography with optimized surface chemistry – the dependable choice for reproducible size exclusion and ion exchange separations.
The product line was aquired by Dr. Maisch HPLC GmbH in 2016 from Grace / Alltech.
| Particle Sizes | 1.9 – 7 µm |
|---|---|
| Phases/Modifications | Diol, SAX, SCX, Si, WCX |
| Pore Sizes | 60 – 800 Å |
Spherical silica-based stationary phases for SEC and IEX
High mechanical stability and reproducibility for long column lifetime
Controlled pore structure for accurate size-based separations
Available with various ionic surface modifications for IEX applications
Suitable for analytical and preparative chromatography
Manufactured by Dr. Maisch HPLC GmbH under strict QC standards
Molecular weight characterization of polymers and biopolymers (SEC)
Separation and purification of proteins, peptides, and nucleic acids (IEX)
Method development for macromolecular and charged analytes
Quality control and research in polymer, biotechnological, and pharmaceutical industries
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| Macrosphere 300 SAX | 5 µm | 300 Å | |
| Macrosphere 300 SCX | 5 µm | 300 Å | |
| Macrosphere 300 WCX | 7 µm | 300 Å | |
| Macrosphere 60 SEC | 5 µm | 60 Å | |
| Macrosphere 60 Si | 5 µm | 0 % | 60 Å |
| Macrosphere 100 SEC | 5 µm | 100 Å | |
| Macrosphere 100 Si | 3 – 5 µm | 0 % | 100 Å |
| Macrosphere 200 SEC | 1.9 – 5 µm | 200 Å | |
| Macrosphere 300 SEC | 5 µm | 300 Å | |
| Macrosphere 400 SEC | 5 µm | 400 Å | |
| Macrosphere 800 SEC | 5 µm | 800 Å |