The ReproSil-Pur 200 series is based on high-purity spherical silica particles with a 200 Å pore size, specifically designed for the separation of biomolecules and large pharmaceutical compounds. The wider pore structure ensures efficient mass transfer and excellent accessibility for peptides, proteins, and other high-molecular-weight analytes, while maintaining outstanding mechanical stability and reproducibility.
ReproSil-Pur 200 phases are available with different surface chemistries to meet diverse analytical requirements. The C18 modification provide strong hydrophobic retention for reversed-phase chromatography, whereas the unmodified silica variant is ideal for normal-phase separations and adsorption-based methods. Together, these phases offer flexibility for a wide range of biotechnological, pharmaceutical, and biochemical applications.
The ReproSil-Pur 200 series combines high-purity silica with larger pore accessibility – the reliable choice for laboratories analyzing peptides and medium biomolecules by HPLC.
| Particle Sizes | 1.9 – 50 µm |
|---|---|
| Phases/Modifications | C18, Diol, Si |
| Pore Sizes | 200 Å |
| Surface Areas | 200 m²/g |
High-purity silica with 200 Å pore size for peptides and medium biomolecules
Available as C18, Diol, and Si phases for different separation mechanisms
Enhanced accessibility for larger analytes compared to 120 Å phases
Excellent reproducibility and sharp peak symmetry
Robust bonding for long column lifetimes
Peptide mapping and separations in biopharmaceutical analysis
Biochemical testing of small proteins and biomolecules
Pharmaceutical impurity profiling under reversed-phase conditions
Food and beverage analysis of peptides, proteins, and additives
Method development requiring pore sizes suitable for medium biomolecules
| Name | Particle Sizes | Carbon Load | Pore Size |
|---|---|---|---|
| ReproSil-Pur 200 C18-AQ | 1.9 – 50 µm | 11 % | 200 Å |
| ReproSil-Pur 200 Diol | 1.9 – 5 µm | 5 % | 200 Å |
| ReproSil-Pur 200 ODS-3 | 3 – 15 µm | 12 % | 200 Å |
| ReproSil-Pur 200 Si | 3 – 5 µm | 0 % | 200 Å |