MySep nano-LC columns are designed as a high-quality alternative to PepSep columns, offering excellent performance, flexibility, and compatibility for modern LC–MS proteomics workflows. Available in inner diameters of 75 µm, 100 µm, and 150 µm, and in lengths from 50 mm up to 500 mm, MySep provides the versatility needed for both high-sensitivity nano-flow and higher-capacity micro-flow applications. These columns deliver high resolution, sharp peak shapes, and consistent retention, making them ideal for peptide mapping, bottom-up proteomics, biomarker discovery, and targeted MS quantification.
A key advantage of the MySep design is the hybrid inlet/outlet connection system. The inlet features a female coupling, suitable for Thermo Fisher Viper and IDEX MarvelXACT connections, allowing seamless integration into existing nano-LC setups. The outlet side uses a male 1/16” PEEK fingertight fitting, providing a secure, leak-free, and dead-volume-free connection. This PEEK fingertight system prevents capillary slippage, simplifies installation, and ensures optimal stability even under high-pressure conditions—critical for reproducible nano-ESI MS performance.
| ReproSil-Pur 120 C18-AQ, 1.9 µm | r119.aq.fm |
|---|---|
| ReproSil Saphir 100 C18, 1.5 µm | ra115.9e.fm |
| Exsil Mono 100 C18, 1.35 µm | 6136973.fm |
| Exsil Mono 100 C18, 1.5 µm | 5136782.fm |
| Exsil Mono 100 C18, 1.7 µm | 6136847.fm |
MySep columns are available packed with premium sub-2 µm C18 materials, including ReproSil-Pur 120 C18-AQ (1.9 µm) for enhanced retention of hydrophilic peptides, ReproSil Saphir 100 C18 (1.5 µm) for maximum separation efficiency, and Exsil Mono 100 C18 (1.35–1.7 µm) for outstanding batch-to-batch reproducibility thanks to monodisperse particle technology. These packings deliver exceptionally high plate counts, excellent peak capacity, and stable chromatographic behavior in long gradients commonly used in proteomics.
By minimizing dead volumes and ensuring reliable mechanical stability, the MySep hardware design supports optimal ionization efficiency in nano-ESI MS workflows. Its adaptable inlet interface makes MySep ideal for laboratories using Viper or MarvelXACT systems, while the robust fingertight outlet ensures leak-free operation and easy handling.
MySep nano-LC columns are available in all combinations of 75 µm, 100 µm, and 150 µm IDs and 50–500 mm lengths, providing full flexibility for method development, ultra-high-resolution separations, or high-throughput workflows. Columns can be ordered as single units, 4-packs, or 10-packs to support routine laboratory operation as well as large-scale proteomics studies. MySep offers a reliable, high-performance solution for any laboratory seeking advanced and hardware-flexible nano-LC columns.
