NanoFusion CSX nano-LC columns are engineered to deliver exceptional chromatographic performance, high sensitivity, and long-term stability for demanding LC–MS and LC–MS/MS proteomics workflows. With available inner diameters of 50 µm, 75 µm, 100 µm, and 150 µm and column lengths ranging from 50 mm to 500 mm, the CSX series offers broad flexibility for nano-flow, micro-flow, high-resolution, and high-throughput applications. Each column features a precision-machined female inlet compatible with Thermo Viper and IDEX MarvelXACT connections, ensuring reliable, low-dispersion UHPLC coupling. NanoFusion CSX is also a highly capable alternative to Aurora CSI columns from IonOpticks.
The hardware design of NanoFusion CSX integrates a captive spray emitter at the column outlet, creating a stable, zero-dead-volume electrospray interface. This configuration enhances ionization efficiency, provides superior signal stability, and supports highly reproducible quantitative and qualitative MS workflows — even during extended gradient runs. The captive spray architecture eliminates alignment challenges, reduces variability, and simplifies system setup, making it ideal for laboratories focused on sensitivity and robustness.
| ReproSil-Pur 120 C18-AQ, 1.9 µm | r119.aq.fcs |
|---|---|
| ReproSil Saphir 100 C18, 1.5 µm | ra115.9e.fcs |
| Exsil Mono 100 C18, 1.35 µm | 6136973.fcs |
| Exsil Mono 100 C18, 1.5 µm | 5136782.fcs |
| Exsil Mono 100 C18, 1.7 µm | 6136847.fcs |
Packed with high-efficiency sub-2 µm C18 materials, NanoFusion CSX columns deliver excellent separation performance with consistent retention times, narrow peak widths, and high peak capacity. This ensures reliable detection and quantification across complex peptide mixtures in workflows such as deep proteome analysis, immunopeptidomics, single-cell proteomics, DIA/SWATH, and targeted MS assays. The rigid capillary construction further supports stable operation under UHPLC pressures, reducing band broadening and maximizing reproducibility.
The stable and resilient column architecture supports reliable operation even at elevated UHPLC pressures. In combination with the captive spray emitter, NanoFusion CSX columns offer a highly consistent, easy-to-use solution for laboratories seeking optimal sensitivity and resolution in demanding proteomics workflows.
Available in all combinations of 50 – 150 µm ID and 50 – 500 mm lengths, the NanoFusion CSX platform allows precise tuning of chromatographic conditions for concentration-limited samples, long-gradient separations, or higher-throughput methods. Columns are offered as single units, 4-packs, or 10-packs to support both routine laboratory needs and intensive research environments. With its advanced emitter design and modern connection architecture, NanoFusion CSX delivers the performance, sensitivity, and reliability required for leading-edge proteomic analysis.
