Adsorbosphere XL NH2

Long-Life Aminopropyl Phase for HILIC, Normal Phase, and Sugar Analysis

Adsorbosphere XL NH2 is a high-purity, fully porous silica-based HPLC column functionalized with aminopropyl (NH₂) groups and optimized for extended lifetime performance. As part of the XL series, it offers improved chemical stability and reduced degradation over long usage periods — especially in aqueous-organic HILIC systems or non-polar solvents.

The NH₂ functionality provides polar retention, weak anion exchange characteristics, and selective interactions for carbohydrates and sugar alcohols, making this phase particularly useful in food, pharmaceutical, and bioanalytical applications.

Adsorbosphere XL NH2 offers extended column stability for polar compound separations — ideal for demanding HILIC and carbohydrate applications requiring high reproducibility and long-term performance.

Key Features

  • Aminopropyl-modified silica (USP L8) bonding for longer lifetime

  • Compatible with HILIC, normal-phase, and carbohydrate analysis

  • Retains polar analytes via hydrogen bonding and dipole interactions

  • Improved chemical robustness in aqueous and high-pH conditions

  • Available in 3 µm, 5 µm and 10 µm particle sizes

  • Fully porous silica with excellent reproducibility

Typical Applications

  • Monosaccharides, oligosaccharides, and sugar alcohols

  • Organic acids and polar drug metabolites

  • Normal-phase separation of polar and neutral compounds

  • Carbohydrate profiling in food and pharmaceutical products

  • HILIC applications with acetonitrile-rich mobile phases

Specifications

Request Product
Particle Sizes
  • 3 µm
  • 5 µm
  • 10 µm
Pore Size 100 Å
Surface Area 200 m²/g
Phase/Modification NH2
Particle Type
Separation Modes
USP Code
Endcapping No
Carbonload 5 %

Applications

0 min8 min
Acids, Bases and Neutrals
µm (100 x 4.6 mm)
0 min15 min
Alcohols, Aldehydes, Ketones
µm (125 x 4 mm)
Analysis of Aflatoxins by HPLC
5 µm (125 x 4 mm)
0 min25 min
Analysis of Alcohol Sugars
µm (250 x 8 mm)
0 min20 min
Analysis of Alcohols and Sugars
µm (250 x 8 mm)
0 min20 min
Analysis of Aromatic Acids
µm (250 x 8 mm)
Analysis of Benzoates
5 µm (125 x 2 mm)
0 min15 min
Analysis of Glycols by HPLC
µm (250 x 8 mm)
0 min8 min
Analysis of Organic Acids
µm (250 x 8 mm)
Analysis of Saccharides by HPLC
µm (250 x 8 mm)
Analysis of Vancomycin by HPLC
µm (250 x 4 mm)
0 min3 min
Anti-Convulsants
µm (150 x 4.6 mm)
0 min20 min
Antineoplastics and related compounds
µm (150 x 4 mm)
Benzodiazepine
µm (33 x 4.6 mm)
0 min20 min
Carbamate and Urea Pesticides
µm (150 x 4.6 mm)
0 min40 min
Coenzyme Q10
µm (250 x 4.6 mm)
0 min40 min
Color Additives - Dyes
µm (150 x 4 mm)
Crude Drugs - Glycosides
µm (250 x 4 mm)
0 min25 min
Determination of Glycerol and Dihydroxyacetone
µm (250 x 8 mm)
0 min15 min
Determination of Lactose and Lactic Acid
µm (250 x 8 mm)
0 min10 min
Determination of Organic Acids in Trichomonas foetus extract
µm (250 x 8 mm)
0 min8 min
Drug Analysis (Antidepressives) by HPLC
µm (125 x 4 mm)
0 min12 min
Egg Yolk Phospholipids
µm (100 x 4.6 mm)
Explosive Analysis, EPA Method 8330
µm (150 x 4.6 mm)
0 min50 min
Fatty Acid Analysis by HPLC - p-Bromophenacylesters of Fatty Acids
µm (250 x 4 mm)
Gold-Turbo 100 Si
µm (33 x 4.6 mm)
0 min0,2 min0,4 min0,6 min0,8 min1 min
Gold-Turbo ODS 4
µm (33 x 4.6 mm)
HPLC Analysis of Coffee
µm (75 x 3 mm)
0 min12 min
HPLC Separation of Antibiotics
µm (125 x 4 mm)
0 min35 minBefore SPEConcentrationAfter SPEConcentration
Nitroaromatics and Naphthols from Soil
µm (150 x 4.6 mm)
0 min30 min
Nucleosides and Bases
µm (250 x 4 mm)
0 min7 min
Organic Acids
µm (250 x 4.6 mm)
0 min14 min
Organochlorine Pesticides
µm (150 x 4.6 mm)
0 min20 min
Peptides and Proteins
µm (150 x 4 mm)
0 min14 min
Phenols
µm (250 x 4.6 mm)
0 min 2 min4 min
Phenols and Chlorophenols
µm (150 x 4.6 mm)
0 min10 min5 min
Polarity mixture
µm (150 x 4.6 mm)
0 min15 min
Saccharides Analysis
µm (250 x 8 mm)
Selectivity of GROM-Sapphire Packings (M.Arangio, H.Engelhardt)
µm (125 x 2 mm)
0 min5 min
Separation of n-Alkylbenzenes
µm (125 x 2 mm)
0 min15 min
Separation of Organic Acids
µm (250 x 8 mm)
0 min12 min
Separation of Organic Acids by Reversed Phase HPLC
µm (125 x 4 mm)
0 min20 min
Separation of Sugars and Alcohols by HPLC
µm (250 x 8 mm)
0 min28 min1 Injection500 Injection1000 Injection
Stability of GROM-Sapphire Packings (Daldrup)
µm (125 x 4 mm)
Standards Mix
µm (150 x 4.6 mm)
0 min20 min
Sugar Analysis
µm (250 x 8 mm)
Vitamins D2 & D3
µm (150 x 4.6 mm)
0 min20 min
Water-soluble Vitamins
µm (150 x 4 mm)
0 min16 min
Xylole (Dimethylbenzene) Isomers
µm (250 x 4 mm)

Material Numbers

Phase Material Number
Adsorbosphere XL NH2, 3 µm 5200058
Adsorbosphere XL NH2, 5 µm 5200059
Adsorbosphere XL NH2, 10 µm 5200060
Adsorbosphere XL Carbohydrate AX Adsorbosphere XL SAX

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