Macrosphere 100 SEC

Silica-Based Diol Phase for Aqueous Size Exclusion Chromatography

Macrosphere 100 SEC is a silica-based stationary phase designed for aqueous size exclusion chromatography (SEC) in the molecular weight range of 500 to 350,000 Daltons. Based on high-purity spherical silica with a 100 Å pore size, this phase features a hydrophilic diol surface modification that ensures highly reproducible and accurate size-based separations of peptides, proteins, and other water-soluble biomolecules.

The diol functionality provides a stable and hydrophilic surface that effectively suppresses secondary interactions between analytes and the silica matrix. This ensures true size exclusion behavior and excellent column performance, even for polar or slightly hydrophobic compounds. The controlled pore structure offers optimal diffusion and mass transfer properties, resulting in sharp peak shapes and consistent molecular weight calibration. With its robust silica backbone, long column lifetime, and reproducible separation performance, Macrosphere 100 SEC is ideally suited for analytical and preparative applications in biochemical, pharmaceutical, and biotechnological research.

Macrosphere 100 SEC combines a hydrophilic diol surface with precision-engineered silica – the reliable choice for accurate and reproducible aqueous SEC separations in the 500 to 350,000 Dalton range.