Macrosphere 60 SEC

Silica-Based Diol Phase for Aqueous Size Exclusion Chromatography

Macrosphere 60 SEC is a silica-based stationary phase specifically engineered for aqueous size exclusion chromatography (SEC) in the molecular weight range of 250 to 28,000 Daltons. The phase is built on high-purity spherical silica with a 60 Å pore size and features a hydrophilic diol surface modification, ensuring accurate size-based separations of small biomolecules and water-soluble polymers.

The diol functionalization forms a stable, hydrophilic surface that minimizes secondary interactions between analytes and the silica matrix, resulting in pure size-exclusion behavior. This design provides high resolution and reproducibility for the separation of peptides, oligosaccharides, small biopolymers, and other low-molecular-weight compounds. With its excellent mechanical stability, consistent calibration behavior, and long operational lifetime, Macrosphere 60 SEC is ideally suited for analytical and preparative use in biochemical, pharmaceutical, and biotechnological applications.

Macrosphere 60 SEC combines a hydrophilic diol-modified surface with precision-engineered silica – the reliable choice for accurate and reproducible aqueous SEC separations in the 250 to 28,000 Dalton range.

Key Features:

  • Silica-based stationary phase with 60 Å pore size and diol surface modification

  • Designed for aqueous SEC separations between 250 and 28,000 Da

  • Hydrophilic surface minimizes adsorption and secondary interactions

  • High mechanical stability and reproducibility for long column lifetime

  • Suitable for analytical and preparative workflows

  • Available particle size: 5 µm

Typical Applications:

  • Molecular weight determination of peptides, oligosaccharides, and small biopolymers

  • Separation of water-soluble compounds in biochemical and pharmaceutical research

  • Characterization of low-molecular-weight biomolecules in aqueous SEC

  • Quality control and method development in life science laboratories

Specifications

Request Product
Particle Size
  • 5 µm
Pore Size 60 Å
Phase/Modification Diol
Particle Type
Separation Mode
USP Code
Endcapping No

Material Number

Phase Material Number
Macrosphere 60 SEC, 5 µm ms05.sec.
Macrosphere 60 Si

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