Platinum 300 C8-EPS

C8 Phase with Extended Polar Selectivity for Reversed-Phase HPLC

Platinum 300 C8-EPS is a silica-based HPLC stationary phase with an octyl bonded surface and a 300 Å pore size, developed for the reversed-phase separation of biomolecules such as peptides, proteins, and other high-molecular-weight compounds. The EPS (Extended Polar Selectivity) design introduces controlled residual silanol activity, enhancing interactions with polar sites of analytes and providing superior selectivity for complex biomolecular separations.

Unlike fully endcapped C8 materials, Platinum 300 C8-EPS is not endcapped, offering a carefully balanced combination of hydrophobic and polar retention. This enables improved resolution for analytes containing both polar and nonpolar functional groups, while maintaining excellent peak shapes and reproducibility. The large 300 Å pore structure ensures optimal accessibility and mass transfer for macromolecules, making this phase ideal for biopharmaceutical and proteomic applications. Built on high-purity, mechanically robust silica, Platinum 300 C8-EPS provides reliable performance, long column lifetime, and consistent results even under demanding gradient conditions.

Platinum 300 C8-EPS delivers the perfect balance between hydrophobic retention and polar selectivity – the dependable choice for accurate and reproducible biomolecular separations in reversed-phase HPLC.

Key Features:

  • Silica-based stationary phase with C8 bonding and 300 Å pore size

  • Not endcapped – engineered for extended polar selectivity (EPS)

  • Balanced hydrophobic and polar retention behavior

  • Optimized for peptides, proteins, and other biomolecules

  • High-purity silica ensures robustness, reproducibility, and long lifetime

  • Available particle sizes: 3 µm, 5 µm

Typical Applications:

  • Reversed-phase separation of peptides and proteins

  • Analysis of biomolecules and biopharmaceutical products

  • Method development requiring both polar and hydrophobic selectivity

  • Quality control and research in pharmaceutical and life science laboratories

Specifications

Request Product
Particle Sizes
  • 3 µm
  • 5 µm
Pore Size 300 Å
Surface Area 100 m²/g
Phase/Modification C8-EPS
Particle Type
Separation Mode
USP Codes
Endcapping No

Material Numbers

Phase Material Number
Platinum 300 C8-EPS, 3 µm 5136694
Platinum 300 C8-EPS, 5 µm 5136695
Platinum 300 C8 Platinum 300 C18

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