Reprobond CN

Polar HPLC Column for Versatile Normal-Phase and Reversed-Phase Separations

Reprobond CN is a silica-based HPLC column with a bonded cyano phase, offering versatile performance in both normal-phase and reversed-phase chromatography. The moderately polar cyano groups provide balanced retention characteristics, combining dipole–dipole interactions with weaker hydrophobic retention compared to C18 or C8 phases. This dual functionality makes Reprobond CN an excellent complementary column for method development, providing orthogonal selectivity in pharmaceutical, biochemical, and environmental applications.

Reprobond CN provides versatile retention characteristics and robust performance – the reliable choice for laboratories requiring flexible normal-phase and reversed-phase HPLC separations.

Key Features:

  • Silica-based stationary phase with cyano (CN) bonded chemistry

  • Suitable for both reversed-phase and normal-phase chromatography

  • Combines polar interactions with moderate hydrophobic retention

  • Robust bonding ensures reproducibility and column longevity

  • Available particle size: 10 µm

Typical Applications:

  • Pharmaceutical impurity profiling with complementary selectivity to C18

  • Fast separations of moderately hydrophobic and polar analytes

  • Normal-phase analysis of nonpolar compounds in chemical and petrochemical testing

  • Method development requiring orthogonal selectivity

Specifications

Request Product
Particle Size
  • 10 µm
Pore Size 120 Å
Surface Area 300 m²/g
Phase/Modification CN
Particle Type
Separation Modes
USP Code
Endcapping Yes
Carbonload 7 %
pH Range 2 to 8

Material Number

Phase Material Number
Reprobond CN, 10 µm rb20.ce.
Reprobond C18 Reprobond NH2

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