ReproSil-Pur 120 RP18-NE is a silica-based HPLC column with octadecyl bonded chemistry on high-purity 120 Å silica. Unlike endcapped phases, this non-endcapped modification retains residual silanol activity, providing enhanced retention and selectivity for polar and basic analytes while still delivering strong hydrophobic interactions for nonpolar compounds.
The 120 Å pore size is optimized for small to medium-sized molecules, ensuring efficient separations with reproducible retention. ReproSil-Pur 120 RP18-NE is particularly suited for pharmaceutical and biochemical laboratories that require complementary selectivity to fully endcapped C18 phases.
ReproSil-Pur 120 RP18-NE provides strong hydrophobic retention with additional polar selectivity – the dependable choice for laboratories requiring complementary reversed-phase HPLC separations.
High-purity silica with octadecyl (C18) bonded chemistry
Non-endcapped (NE) phase retains silanol activity for polar interactions
120 Å pore size optimized for small to medium-sized molecules
Dual retention: hydrophobic plus polar interactions
Available particle sizes: 3 µm, 5 µm
Pharmaceutical impurity profiling with enhanced polar selectivity
Biochemical separations of basic drugs, polar metabolites, and small biomolecules
Food and beverage analysis of polar and amphiphilic compounds
Environmental monitoring of pollutants requiring mixed retention mechanisms
Method development requiring orthogonal selectivity to endcapped C18 phases
| Particle Sizes |
|
|---|---|
| Pore Size | 120 Å |
| Surface Area | 300 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| Carbonload | 14 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil-Pur 120 RP18-NE, 3 µm | r13.90. |
| ReproSil-Pur 120 RP18-NE, 5 µm | r15.90. |