ReproSil Saphir 100 C4

Short-Chain C4 Column for Gentle Reversed-Phase Biomolecule Separations

ReproSil Saphir 100 C4 from Dr. Maisch HPLC GmbH is a high-purity silica-based HPLC column with a 100 Å pore size and a butyl bonded phase. The short alkyl chain provides reduced hydrophobic retention compared to C18 and C8, allowing mild elution conditions that help preserve the structural integrity of peptides, small proteins, and other sensitive biomolecules. The ultra-pure silica base ensures high efficiency, excellent peak symmetry, and reproducible performance in both analytical and method development workflows.

ReproSil Saphir 100 C4 columns from Dr. Maisch HPLC GmbH deliver gentle yet highly efficient separations – the ideal choice for sensitive biomolecule chromatography in reversed-phase HPLC.

Key Features:

  • Fully porous, ultra-pure silica with 100 Å pore size

  • C4 bonding – USP L26 classification

  • Lower hydrophobic retention for gentle biomolecule analysis

  • Excellent reproducibility and peak symmetry

  • Manufactured and QC-tested by Dr. Maisch HPLC GmbH

  • Available in 5 µm and 10 µm particle sizes

Typical Applications:

  • Peptide and small protein separations under mild conditions

  • Biopharmaceutical QC and proteomics workflows

  • Gradient HPLC of hydrophobic biomolecules with reduced retention

  • Method development for sensitive analytes

Specifications

Request Product
Particle Sizes
  • 5 µm
  • 10 µm
Pore Size 100 Å
Surface Area 400 m²/g
Phase/Modification C4
Particle Type
Separation Mode
USP Code
Endcapping Yes
Carbonload 8 %
pH Range 2 to 8

Material Numbers

Phase Material Number
ReproSil Saphir 100 C4, 5 µm ra15.4e.
ReproSil Saphir 100 C4, 10 µm ra10.4e.
ReproSil Saphir 100 C8

Any questions?
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info@dr-maisch.com +49 7073 5035 7

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