ReproSil 100 C4 is a reversed-phase HPLC column from Dr. Maisch HPLC GmbH, packed with ultra-pure, fully porous spherical silica (100 Å pore size) functionalized with butyl ligands. The short alkyl chains provide mild hydrophobic retention, making this phase particularly suitable for peptides, small proteins, and hydrophobic biomolecules where reduced retention compared to C18 or C8 is advantageous. With its balanced retention characteristics and high reproducibility, ReproSil 100 C4 is widely used in bioanalytical workflows, proteomics, and pharmaceutical research for the analysis of biomolecules requiring gentle separation conditions.
ReproSil 100 C4 from Dr. Maisch HPLC GmbH offers reliable and reproducible short-chain reversed-phase performance – the ideal solution for laboratories analyzing peptides and small biomolecules.
C4 reversed-phase silica – USP L26
100 Å pore size optimized for peptides and small proteins
Mild hydrophobic retention for improved recovery of sensitive analytes
High column efficiency and reproducibility
Manufactured and QC-tested by Dr. Maisch HPLC GmbH
Available in 3 µm, 5 µm, and 10 µm particle sizes
Reversed-phase HPLC of peptides and small proteins
Bioanalytical LC-MS of biomolecules with moderate hydrophobicity
Proteomics and peptide mapping workflows
Method development where C18 or C8 phases are too retentive
| Particle Sizes |
|
|---|---|
| Pore Size | 100 Å |
| Surface Area | 280 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 5 % |
| pH Range | 2 to 8 |
| Phase | Material Number |
|---|---|
| ReproSil 100 C4, 3 µm | r13.46. |
| ReproSil 100 C4, 5 µm | r15.46. |
| ReproSil 100 C4, 10 µm | r10.46. |