Amino Acid Analysis employing Precolumn Derivatisation with FMOC followed by Pentane Extraction

1 Cys-SO₃H
2 Asp
3 Ser
4 Glu
5 Thr
6 Gly
7 Arg
8 Ala
9 Tyr
10 Pro
11 Met
12 FMOC-OH
13 Val
14 Nor-Val
15 Phe
16 Ile
17 Leu
18 His
19 Lys
Graph of Amino Acid Analysis employing Precolumn Derivatisation with FMOC followed by Pentane Extraction with ,  µm (250 x 4.6 mm)
Substances 1 Cys-SO₃H
2 Asp
3 Ser
4 Glu
5 Thr
6 Gly
7 Arg
8 Ala
9 Tyr
10 Pro
11 Met
12 FMOC-OH
13 Val
14 Nor-Val
15 Phe
16 Ile
17 Leu
18 His
19 Lys
Eluent A: 50 mM Na-acetate, pH 4.2 / ACN = 80 / 20 B: 50 mM Na-acetate, pH 4.2 / ACN = 20 / 80
Flow Rate 1 ml / min
Detection 263 nm exc., 313 nm em

Application for Column

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