HPLC of Steroids

1 Progesterone
2 Estrone
3 ß-Estradiol
4 Ethynylestradiol
5 Prednisolone
Graph of HPLC of Steroids with ,  µm (250 x 4 mm)
Substances 1 Progesterone
2 Estrone
3 ß-Estradiol
4 Ethynylestradiol
5 Prednisolone
Eluent EtOH / n-hexane = 5 / 95
Flow Rate 1.5 ml / min
Detection 220 nm

Application for Column

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