Hydrophobic Interaction Chromatography of Proteins

1 Cytochrome C
2 Myoglobin
3 β-Lactoglobulin
4 Ribonuclease A
5 Lysozyme
6 α-Chymotrypsin
7 Chymotrypsinogen
Graph of Hydrophobic Interaction Chromatography of Proteins with ,  µm (250 x 4.6 mm)
Substances 1 Cytochrome C
2 Myoglobin
3 β-Lactoglobulin
4 Ribonuclease A
5 Lysozyme
6 α-Chymotrypsin
7 Chymotrypsinogen
Eluent A: 2.0 M (NH₄)₂SO₄ - 0.1 M KH₂PO₄, pH 6.8 B: 0.1 M KH₂PO₄, pH 6.8
Flow Rate 1.0 ml / min
Detection 280 nm

Application for Column

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