Isocratic HPLC Separation of Sorbic, Benzoic & Ascorbic Acids – Dr. Maisch

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Graph of Isocratic HPLC Separation of Sorbic, Benzoic & Ascorbic Acids with Exsil Pure 120 SAX, 3 µm (250 x 4.6 mm)

Substances	1 Sorbic Acid 2 Benzoic Acid 3 Ascorbic Acid
Eluent	AcN-(50mM NH₄HPO₄) 45:55
Flow Rate	1.5 mL / min
Detection	230 nm and 260 nm

Application for Column

Exsil Pure 120 SAX Particle Size: 3 µm
Column Size: 250 × 4.6 mm
Pore Size: 120 Å

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