| Substances |
1
Clavulanic acid
( 2.5 min)
2 Amoxicillin ( 4.3 min) |
|---|---|
| Eluent | Dissolve 7.8 gm Sodium di-hydrogen in 1L water. Adjust pH to 4.4 with dilute ortho-phosphoric acid. Mix buffer & methanol 95:5 (v/v) |
| Flow Rate | 2.0 ml / min |
| Detection | 220 nm |