Allsphere C1 is a reversed-phase HPLC column functionalized with C1 groups, offering the lowest hydrophobicity within the Allsphere product family. Designed for analytes that elute too late on traditional C18 or C8 columns, this phase enables shorter retention times and better control over strongly retained or very large molecules.
Its minimal carbon load makes Allsphere C1 especially useful in gradient optimization, early elution of hydrophobic compounds, or the separation of macromolecules where excessive retention must be avoided.
Allsphere C1 is the go-to phase when minimal hydrophobic retention is required — offering precise elution control for early-eluting or large molecules in reversed-phase HPLC.
C1 bonded phase (USP L13)
Very low hydrophobicity – ideal for early eluting analytes
Reduced retention for large or lipophilic compounds
Compatible with reversed-phase mobile phases (ACN, MeOH, buffers)
Based on fully porous, high-purity silica
Available in 3 µm, 5 µm and 10 µm particle sizes
Separation of lipophilic compounds with high retention on C18
Gradient shortening and method speed optimization
Large biomolecules and neutral compounds
Screening methods requiring minimal retention
Reversed-phase method development with reduced hydrophobic interaction
| Particle Sizes |
|
|---|---|
| Pore Size | 80 Å |
| Surface Area | 220 m²/g |
| Phase/Modification | C1 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 3.5 % |
| Phase | Material Number |
|---|---|
| Allsphere C1, 3 µm | 5203009 |
| Allsphere C1, 5 µm | 5203010 |
| Allsphere C1, 10 µm | 5203011 |