Allsphere C6

Medium-Hydrophobicity Reversed-Phase Column for Balanced Retention

Allsphere C6 is a reversed-phase HPLC column functionalized with hexyl (C6) groups on fully porous, high-purity silica. Positioned between C8 and C4 in hydrophobicity, this phase offers moderate retention for non-polar compounds and balanced selectivity for analytes that are either too strongly or too weakly retained on other alkyl phases. Allsphere C6 is especially suitable for method development, impurity profiling, and applications that require fine-tuned retention behavior.

Allsphere C6 offers flexible reversed-phase performance for compounds requiring moderate hydrophobic interaction – ideal for fine-tuning retention in challenging separations.

Key Features

  • Hexyl (C6) bonded phase with intermediate hydrophobicity

  • Fully porous silica with consistent surface properties

  • Balanced retention strength for method flexibility

  • Compatible with aqueous-organic mobile phases (e.g. MeOH, ACN)

  • Available in standard particle sizes (e.g. 3 µm, 5 µm, 10 µm)

Typical Applications

  • Reversed-phase separations of moderately hydrophobic analytes

  • Pharmaceutical method development and impurity screening

  • Food components, preservatives, and formulation ingredients

  • Selectivity screening between C4/C8 during method optimization

Specifications

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Particle Sizes
  • 3 µm
  • 5 µm
  • 10 µm
Pore Size 80 Å
Surface Area 220 m²/g
Phase/Modification C6
Particle Type
Separation Mode
USP Code
Endcapping Yes
Carbonload 9 %

Material Numbers

Phase Material Number
Allsphere C6, 3 µm 5203012
Allsphere C6, 5 µm 5203013
Allsphere C6, 10 µm 5203014
Allsphere C1 Allsphere C8

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