Exsil 300 Butyl is a wide-pore C4 silica stationary phase engineered for demanding reversed-phase HPLC applications involving proteins, peptides, and other high-molecular-weight analytes. Built on 300 Å pore size silica with a butyl (C4) bonded surface, this phase offers moderate hydrophobic retention, making it ideal for biomolecules that require balanced interactions without excessive retention time.
The wide-pore structure ensures excellent accessibility and efficient mass transfer for large molecules, delivering sharp peak shapes, high resolution, and reliable reproducibility. The moderate hydrophobicity of the C4 surface provides effective retention of peptides and small proteins while minimizing denaturation and promoting high recovery – a major advantage in biopharmaceutical, proteomic, and life-science workflows. Exsil 300 Butyl is part of the Exsil 300 family, known for its uniform spherical particles, robust mechanical strength, and compatibility with high pressures. Its reproducible performance and flexible selectivity profile make it a strong choice for method development and routine analysis where reliable reversed-phase separations of large or sensitive analytes are required.
Exsil 300 Butyl combines wide-pore accessibility with balanced hydrophobic interactions – the reliable choice for reproducible, high-efficiency reversed-phase separations of peptides, proteins, and other biomolecules.
Wide-pore (300 Å) silica optimized for proteins, peptides, and large biomolecules
Butyl (C4) bonded phase with moderate hydrophobic retention
Excellent accessibility and mass transfer for high-molecular-weight analytes
Stable performance across gradient and isocratic conditions
High column efficiency, sharp peak shapes, and reproducible results
Reversed-phase HPLC separation of proteins, peptides, and biopolymers
Biomolecule profiling and characterization in pharmaceutical research
Method development for moderate hydrophobicity selectivity
Quality control and routine analysis in life-science laboratories
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 3 % |
| pH Range | 2 - 8 |
| Phase | Material Number |
|---|---|
| Exsil 300 Butyl, 3 µm | 5136604. |
| Exsil 300 Butyl, 5 µm | 5136610. |
| Exsil 300 Butyl, 10 µm | 5136618. |