Exsil 300 Hexyl

Wide-Pore C6 Phase for Enhanced Selectivity in Protein and Peptide HPLC

Exsil 300 Hexyl is a high-performance wide-pore (300 Å) silica stationary phase featuring a hexyl (C6) bonded surface, specifically designed for reversed-phase HPLC separations of proteins, peptides, and other large biomolecules. The C6 functionalization provides intermediate hydrophobic retention, positioned between C4 and C8 phases, offering optimized selectivity and controlled elution behavior for complex biological samples.

The 300 Å pore size ensures full accessibility of macromolecules, enabling efficient mass transfer and minimizing steric hindrance. This results in high resolution, sharp peak symmetry, and reproducible retention times, even for larger peptides and small to medium-sized proteins. The balanced hydrophobic interaction of the hexyl chain improves separation of structurally related biomolecules while maintaining high recovery. Exsil 300 Hexyl is manufactured from uniform, spherical high-purity silica, delivering excellent mechanical stability, consistent packing behavior, and long column lifetime. The phase performs reliably under gradient conditions and is fully compatible with common reversed-phase mobile phases, including aqueous/organic mixtures with TFA or formic acid modifiers.

Exsil 300 Hexyl combines wide-pore accessibility with optimized C6 hydrophobic selectivity – the dependable solution for high-resolution, reproducible reversed-phase separations of complex biomolecules.