Exsil 300 ODS is a high-performance 300 Å wide-pore silica stationary phase with a C18 bonded surface, specifically developed for demanding reversed-phase HPLC separations of peptides, proteins, and other high-molecular-weight biomolecules. The long-chain C18 functionalization provides strong hydrophobic retention, enabling high-resolution separations of complex biological samples and structurally related analytes.
The 300 Å pore structure ensures optimal accessibility for large molecules, reducing steric limitations and supporting efficient mass transfer. This results in excellent peak symmetry, high efficiency, and reproducible retention times, even under gradient conditions. Exsil 300 ODS is particularly well suited for applications requiring maximum resolving power and enhanced retention of hydrophobic peptide sequences. Manufactured from high-purity, spherical silica particles, Exsil 300 ODS delivers outstanding mechanical stability, uniform packing characteristics, and long column lifetime. The phase performs reliably in aqueous/organic mobile phases and is fully compatible with common modifiers such as TFA and formic acid, making it ideal for analytical, semi-preparative, and preparative workflows in pharmaceutical and biotech laboratories.
Exsil 300 ODS combines wide-pore accessibility with powerful C18 hydrophobic selectivity – delivering precise, high-resolution, and reproducible reversed-phase separations for advanced peptide and protein analysis.
300 Å wide-pore silica optimized for proteins and large peptides
C18 (ODS) bonded phase for strong hydrophobic retention
High resolution and peak symmetry in gradient reversed-phase HPLC
Excellent mass transfer for high-molecular-weight analytes
Robust spherical silica for reproducible, long-term performance
Suitable for analytical and preparative separations
Reversed-phase HPLC of peptides and proteins
Separation of hydrophobic peptide impurities
Biopharmaceutical method development and validation
Quality control of therapeutic peptides and protein-based drugs
Proteomic and biomolecular analysis
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C18 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 8 % |
| pH Range | 2 - 8 |
| Phase | Material Number |
|---|---|
| Exsil 300 ODS, 3 µm | 5136606. |
| Exsil 300 ODS, 5 µm | 5136612. |
| Exsil 300 ODS, 7 µm | 5136749. |
| Exsil 300 ODS, 10 µm | 5136620. |