Exsil 300 Octyl is a 300 Å wide-pore silica stationary phase featuring an octyl (C8) bonded surface, specifically engineered for demanding reversed-phase HPLC separations of peptides, proteins, and other high-molecular-weight biomolecules. The C8 functionalization provides stronger hydrophobic retention than C4 or C6 phases while maintaining excellent recovery and controlled elution behavior.
The 300 Å pore size ensures full accessibility for large analytes, enabling efficient mass transfer and minimizing steric exclusion effects. This results in high resolution, sharp peak symmetry, and reproducible retention times, even for complex peptide mixtures and small to medium-sized proteins. The balanced hydrophobic interaction of the octyl surface makes Exsil 300 Octyl ideal for applications requiring enhanced selectivity and improved separation of closely related biomolecular variants. Manufactured from high-purity, spherical silica particles, Exsil 300 Octyl offers outstanding mechanical strength, uniform packing performance, and long operational lifetime. The phase performs reliably under gradient reversed-phase conditions and is fully compatible with aqueous/organic mobile phases containing modifiers such as TFA or formic acid.
Exsil 300 Octyl combines wide-pore silica accessibility with optimized C8 hydrophobic selectivity – delivering reliable, high-resolution, and reproducible reversed-phase separations for advanced peptide and protein analysis.
300 Å wide-pore silica optimized for proteins and peptides
Octyl (C8) bonded phase with enhanced hydrophobic retention
Excellent mass transfer and accessibility for large biomolecules
High column efficiency and reproducible chromatographic performance
Robust spherical particles for analytical and preparative HPLC
Ideal for gradient reversed-phase separations
Reversed-phase HPLC of peptides and small to medium-sized proteins
Separation of peptide impurities and closely related biomolecular variants
Biopharmaceutical method development and optimization
Quality control and characterization of therapeutic biomolecules
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C8 |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | Yes |
| Carbonload | 5 % |
| pH Range | 2 - 8 |
| Phase | Material Number |
|---|---|
| Exsil 300 Octyl, 3 µm | 5136605. |
| Exsil 300 Octyl, 5 µm | 5136611. |
| Exsil 300 Octyl, 10 µm | 5136619. |