Exsil 300 PAX is a specialized 300 Å wide-pore silica stationary phase bonded with polyethyleneimine, designed for high-performance anion-exchange chromatography of biomolecules and acidic compounds. The branched polyethylenimine surface provides multiple protonatable amine functionalities, enabling strong electrostatic interactions and high binding capacity for negatively charged analytes.
The 300 Å pore size ensures excellent accessibility for large biomolecules such as proteins, peptides, nucleic acids, and acidic glycoproteins. The combination of wide pores and a high-density PEI layer delivers efficient mass transfer, sharp peak shapes, and reproducible retention behavior – even for complex biological samples. Exsil 300 PAX offers enhanced selectivity for acidic and anionic species through a combination of electrostatic interactions, hydrogen bonding, and secondary polar effects. The high-purity spherical silica matrix provides outstanding mechanical stability, uniform packing, and long column lifetime, making the phase suitable for both analytical and preparative ion-exchange HPLC applications. Fully compatible with aqueous buffer systems and gradient elution using salt or pH control, Exsil 300 PAX is ideal for method development and routine separations in biopharmaceutical, proteomic, and life-science laboratories.
Exsil 300 PAX combines wide-pore silica accessibility with high-capacity polyethyleneimine chemistry – delivering reliable, high-resolution anion-exchange separations for demanding biomolecular applications.
Polyethyleneimine (PEI) bonded phase for strong anion-exchange chromatography
300 Å wide-pore silica optimized for proteins and large biomolecules
High binding capacity for negatively charged analytes
Excellent mass transfer and peak symmetry
Robust spherical silica for reproducible and long-term performance
Compatible with aqueous buffer systems and salt gradients
Anion-exchange separation of proteins and peptides
Purification and analysis of nucleic acids
Separation of acidic biomolecules and glycoproteins
Biopharmaceutical process development and QC
Method development in strong anion-exchange HPLC
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | PEI |
| Particle Type | |
| Separation Modes | |
| Endcapping | No |
| pH Range | 2 - 8 |
| Phase | Material Number |
|---|---|
| Exsil 300 PAX, 3 µm | 5136608. |
| Exsil 300 PAX, 5 µm | 6136878. |
| Exsil 300 PAX, 7 µm | 5136616. |