Exsil 300 SAX is a high-performance 300 Å wide-pore silica stationary phase featuring a strong anion exchange (SAX) functionality, specifically developed for the separation of negatively charged biomolecules and acidic compounds by HPLC. The permanently charged quaternary ammonium groups provide strong and stable electrostatic interactions, ensuring reliable retention of anionic analytes across a broad pH range.
The wide 300 Å pore structure guarantees full accessibility for proteins, peptides, nucleic acids, and other high-molecular-weight species, enabling efficient mass transfer and sharp, symmetrical peak shapes. The combination of high-capacity ion-exchange functionality and wide-pore silica results in excellent resolution and reproducibility, even for complex biological samples. Manufactured from high-purity, spherical silica particles, Exsil 300 SAX offers superior mechanical stability, uniform packing behavior, and long column lifetime. The phase performs reliably under aqueous buffer conditions and supports gradient elution via salt concentration or pH adjustment, making it ideal for analytical, preparative, and process-scale ion-exchange applications.
Exsil 300 SAX combines wide-pore silica accessibility with strong, stable anion-exchange chemistry – delivering precise, reproducible, and high-resolution separations for demanding biomolecular and analytical HPLC applications.
Strong anion exchange (SAX) functionality with quaternary ammonium groups
300 Å wide-pore silica optimized for proteins and large biomolecules
High binding capacity for negatively charged analytes
Stable performance across a broad pH range
Excellent efficiency, peak symmetry, and reproducibility
Robust spherical silica for long-term column stability
Anion-exchange HPLC of proteins and peptides
Separation of nucleotides and nucleic acids
Purification of acidic biomolecules and glycoproteins
Biopharmaceutical process development and quality control
Environmental and analytical separation of anionic compounds
| Particle Size |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | SAX |
| Particle Type | |
| Separation Mode | |
| Endcapping | No |
| pH Range | 2 - 8 |
| Phase | Material Number |
|---|---|
| Exsil 300 SAX, 5 µm | 6136987. |