Exsil 300 Si is a high-purity 300 Å wide-pore silica stationary phase designed for demanding normal-phase chromatography, adsorption chromatography, and HILIC applications. The unmodified silica surface provides strong polar interactions, enabling efficient separation of polar compounds, isomers, carbohydrates, and structurally related analytes.
The 300 Å pore size ensures excellent accessibility for larger molecules, including peptides, biomolecules, and high-molecular-weight compounds. This wide-pore structure improves mass transfer and reduces steric hindrance, resulting in high efficiency, sharp peak shapes, and reproducible retention behavior. Manufactured from uniform, spherical silica particles, Exsil 300 Si delivers outstanding mechanical stability, consistent packing performance, and long column lifetime. The high surface activity of the silica allows selective interactions via hydrogen bonding, dipole–dipole forces, and adsorption mechanisms, making it a versatile solution for method development and routine analytical separations. Exsil 300 Si is fully compatible with standard normal-phase solvent systems (e.g., hexane, isopropanol mixtures) as well as aqueous-organic mobile phases used in HILIC chromatography.
Exsil 300 Si combines wide-pore accessibility with high-purity bare silica selectivity – delivering reliable, efficient, and reproducible separations for polar and adsorption-driven chromatographic applications.
300 Å wide-pore bare silica for enhanced accessibility
Strong polar surface interactions for normal-phase and HILIC
Excellent mass transfer for peptides and larger analytes
High efficiency and reproducible chromatographic performance
Robust spherical particles for analytical and preparative use
Ideal for adsorption-based separations
Normal-phase separation of polar and moderately polar compounds
HILIC analysis of carbohydrates and biomolecules
Isomer separations and adsorption chromatography
Method development in pharmaceutical and chemical laboratories
Analytical and preparative silica-based separations
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | Si |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| Carbonload | 0 % |
| pH Range | 2 - 8 |
| Phase | Material Number |
|---|---|
| Exsil 300 Si, 3 µm | 5136603. |
| Exsil 300 Si, 5 µm | 5136609. |
| Exsil 300 Si, 7 µm | 5136614. |
| Exsil 300 Si, 10 µm | 5136617. |