Macrosphere 300 SAX

Strong Anion Exchange Phase for Biomolecule Separations

Macrosphere 300 SAX is a silica-based strong anion exchange phase designed for the separation and purification of negatively charged biomolecules such as nucleotides, acidic proteins, and peptides. The phase is built on high-purity spherical silica with a 300 Å pore size, providing excellent accessibility for large biomolecules and efficient mass transfer.

Equipped with quaternary ammonium functional groups, Macrosphere 300 SAX offers high ion exchange capacity, strong electrostatic interactions, and outstanding reproducibility under a wide range of buffer conditions. The large-pore silica support ensures robust performance in both analytical and preparative ion exchange chromatography, making it ideal for biopharmaceutical and biochemical applications.

Macrosphere 300 SAX delivers strong anion exchange performance with the accessibility of a large-pore silica support – the reliable choice for high-resolution separation of negatively charged biomolecules.

Key Features:

  • Silica-based strong anion exchange (SAX) phase with 300 Å pore size

  • Optimized for acidic and negatively charged biomolecules

  • High ion exchange capacity and reproducibility

  • Excellent mechanical stability and long column lifetime

  • Suitable for analytical and preparative chromatography

  • Available particle size: 5 µm

Typical Applications:

  • Separation and purification of nucleotides, acidic peptides, and proteins

  • Biopharmaceutical process development and quality control

  • Method optimization for anion exchange chromatography

Specifications

Request Product
Particle Size
  • 5 µm
Pore Size 300 Å
Phase/Modification SAX
Particle Type
Separation Mode
USP Code

Material Number

Phase Material Number
Macrosphere 300 SAX, 5 µm 5207006
Macrosphere 300 SCX

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