Macrosphere 300 WCX

Weak Cation Exchange Phase for Biomolecule Separations

Macrosphere 300 WCX is a silica-based weak cation exchange phase designed for the selective separation of positively charged biomolecules under milder buffer and pH conditions. The phase is built on high-purity spherical silica with a 300 Å pore size, offering excellent pore accessibility and diffusion characteristics for large biomolecules such as peptides and proteins.

Functionalized with weakly acidic carboxylic groups, Macrosphere 300 WCX provides moderate ion exchange strength and fine-tuned selectivity, allowing gentle separations of pH-sensitive compounds or biomolecules prone to denaturation. The robust silica support ensures reproducibility, stability, and long lifetime, even under varying chromatographic conditions.

Macrosphere 300 WCX offers mild and controlled ion exchange selectivity on a stable silica matrix – the ideal choice for reproducible and gentle separation of positively charged biomolecules.

Key Features:

  • Silica-based weak cation exchange (WCX) phase with 300 Å pore size

  • Gentle ion exchange interactions for pH-sensitive biomolecules

  • Fine selectivity control through adjustable buffer and pH conditions

  • High mechanical and chemical stability

  • Suitable for analytical and preparative separations

  • Available particle size: 7 µm

Typical Applications:

  • Separation of peptides and proteins under mild chromatographic conditions

  • Purification of pH-sensitive or labile biomolecules

  • Biopharmaceutical and biochemical research requiring selective cation exchange

Specifications

Request Product
Particle Size
  • 7 µm
Pore Size 300 Å
Phase/Modification WCX
Particle Type
Separation Mode
USP Code

Material Number

Phase Material Number
Macrosphere 300 WCX, 7 µm 5207014
Macrosphere 300 SCX

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