Macrosphere 200 SEC is a silica-based stationary phase developed for aqueous size exclusion chromatography (SEC) in the molecular weight range of 5,000 to 500,000 Daltons. The material is based on high-purity spherical silica with a 200 Å pore size and features a hydrophilic diol surface modification that provides true size-exclusion behavior and excellent reproducibility for medium to large biomolecules.
The diol-functionalized surface effectively suppresses secondary interactions between the analytes and the silica support, ensuring accurate molecular size-based separations under aqueous conditions. The optimized pore structure facilitates efficient diffusion and mass transfer, delivering sharp peak shapes, stable retention, and consistent molecular weight calibration across repeated runs. With its robust mechanical stability, long column lifetime, and high reproducibility, Macrosphere 200 SEC is particularly suited for the analysis and characterization of proteins, polysaccharides, and other biopolymers in biochemical, pharmaceutical, and biotechnological research environments.
Macrosphere 200 SEC combines optimized pore design with a hydrophilic diol surface – the reliable choice for accurate and reproducible aqueous SEC separations in the 5,000 to 500,000 Dalton range.
Silica-based stationary phase with 200 Å pore size and hydrophilic diol modification
Designed for aqueous SEC separations between 5,000 and 500,000 Da
Hydrophilic surface minimizes adsorption and non-size-based interactions
Excellent reproducibility, mechanical stability, and column durability
Suitable for analytical and preparative workflows
Available particle size: 1.9 µm, 5 µm
Molecular weight determination of proteins, polysaccharides, and biopolymers
Aqueous SEC of large biomolecules and macromolecular complexes
Analysis of biopharmaceuticals and high-molecular-weight compounds
Quality control and method development in life science and pharmaceutical laboratories
| Particle Sizes |
|
|---|---|
| Pore Size | 200 Å |
| Phase/Modification | Diol |
| Particle Type | |
| Separation Mode | |
| USP Code | |
| Endcapping | No |
| Phase | Material Number |
|---|---|
| Macrosphere 200 SEC, 1.9 µm | ms219.sec. |
| Macrosphere 200 SEC, 5 µm | ms25.sec. |