Macrosphere 300 SEC

Silica-Based Diol Phase for Aqueous Size Exclusion Chromatography

Macrosphere 300 SEC is a silica-based stationary phase engineered for aqueous size exclusion chromatography (SEC) in the molecular weight range of 7,500 to 1,200,000 Daltons. Built on high-purity spherical silica with a 300 Å pore size, this phase provides excellent accessibility for large biomolecules and ensures accurate, reproducible size-based separations across a broad molecular weight spectrum.

The surface of Macrosphere 300 SEC is modified with hydrophilic diol groups, forming a stable, inert layer that minimizes secondary interactions between analytes and the silica surface. This results in pure size-exclusion behavior and sharp peak shapes, even for high-molecular-weight proteins, polysaccharides, and biopolymers. The optimized pore architecture allows efficient mass transfer and diffusion, enabling precise molecular weight characterization and calibration. With its robust silica backbone, high reproducibility, and long operational lifetime, Macrosphere 300 SEC is ideally suited for biochemical, biotechnological, and pharmaceutical applications, particularly for the analysis and purification of macromolecules and biopharmaceuticals.

Macrosphere 300 SEC combines a large-pore silica structure with a hydrophilic diol surface – the reliable choice for accurate, reproducible, and high-resolution aqueous SEC separations in the 7,500 to 1,200,000 Dalton range.