Macrosphere 800 SEC

Silica-Based Diol Phase for Aqueous Size Exclusion Chromatography

Macrosphere 800 SEC is a silica-based stationary phase designed for aqueous size exclusion chromatography (SEC) in the molecular weight range of 150,000 to 1,250,000 Daltons. The material is based on high-purity spherical silica with an 800 Å pore size and features a hydrophilic diol surface modification, providing true size-exclusion behavior for very large biomolecules and aggregates.

The diol-functionalized surface creates a stable, hydrophilic layer that minimizes secondary interactions and ensures accurate, purely size-dependent separations. The wide pore structure enables excellent diffusion and mass transfer for high-molecular-weight proteins, polysaccharides, and biopolymers, resulting in sharp peak shapes and reliable molecular weight calibration. With its robust silica backbone, high reproducibility, and long operational lifetime, Macrosphere 800 SEC is ideally suited for the analysis and characterization of large biomolecules, protein complexes, and biopharmaceutical formulations in aqueous environments.

Macrosphere 800 SEC combines an ultra-large-pore silica structure with a hydrophilic diol surface – the reliable choice for accurate and reproducible aqueous SEC separations of very high-molecular-weight biomolecules between 150,000 and 1,250,000 Daltons.