Macrosphere 400 SEC

Silica-Based Diol Phase for Aqueous Size Exclusion Chromatography

Macrosphere 400 SEC is a silica-based stationary phase developed for aqueous size exclusion chromatography (SEC) in the molecular weight range of 50,000 to 1,200,000 Daltons. The material is based on high-purity spherical silica with a 400 Å pore size and features a hydrophilic diol surface modification, ensuring pure size-exclusion behavior and reproducible separations of large biomolecules.

The diol-functionalized surface provides a hydrophilic, inert interface that effectively suppresses secondary interactions and adsorption effects. This guarantees reliable, size-dependent separations of high-molecular-weight compounds such as proteins, polysaccharides, and biopolymers. The wide pore structure facilitates efficient mass transfer and diffusion, resulting in sharp peaks and excellent resolution, even for very large macromolecules and aggregates.

With its robust silica backbone, outstanding mechanical stability, and consistent calibration behavior, Macrosphere 400 SEC is ideally suited for both analytical and preparative use in biotechnological, pharmaceutical, and biochemical laboratories focused on the analysis of large biomolecules.

Macrosphere 400 SEC combines a large-pore silica structure with a hydrophilic diol surface – the reliable choice for accurate and reproducible aqueous SEC separations of high-molecular-weight biomolecules in the 50,000 to 1,200,000 Dalton range.