Platinum 300 C18-EPS is a silica-based HPLC stationary phase with an octadecyl bonded surface and a 300 Å pore size, specifically developed for the separation of large biomolecules such as peptides and proteins. The EPS designation stands for Extended Polar Selectivity, indicating a bonding chemistry that enhances retention and selectivity for polar compounds while maintaining strong hydrophobic interactions.
Unlike traditional endcapped C18 materials, Platinum 300 C18-EPS is not endcapped, leaving controlled residual silanol activity on the silica surface. This design provides additional polar interaction sites, resulting in improved resolution for analytes with mixed hydrophobic and polar characteristics. The combination of large pore size and polar selectivity makes this phase particularly effective for biopharmaceutical and biotechnological applications. The high-purity silica matrix ensures excellent mechanical stability, reproducibility, and consistent performance under both isocratic and gradient conditions.
Platinum 300 C18-EPS combines strong hydrophobic retention with extended polar selectivity – the reliable choice for versatile and reproducible separations of complex biomolecules in reversed-phase HPLC.
Silica-based stationary phase with C18 bonding and 300 Å pore size
Not endcapped – designed for extended polar selectivity (EPS)
Balanced retention for polar and hydrophobic analytes
High-purity silica ensures reproducibility and robustness
Suitable for analytical and preparative separations
Available particle sizes: 3 µm, 5 µm, 10 µm
Separation of peptides, proteins, and other biomolecules
Analysis of pharmaceuticals and polar organic compounds
Method development requiring complementary hydrophobic and polar selectivity
Quality control and research in biopharmaceutical and life science laboratories
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C18-EPS |
| Particle Type | |
| Separation Mode | |
| USP Codes | |
| Endcapping | No |
| Phase | Material Number |
|---|---|
| Platinum 300 C18-EPS, 3 µm | 5136700 |
| Platinum 300 C18-EPS, 5 µm | 5136701 |
| Platinum 300 C18-EPS, 10 µm | 5136693 |