Platinum 300 C4-EPS is a silica-based HPLC stationary phase with a butyl bonded surface and a 300 Å pore size, specifically designed for the separation of large biomolecules such as peptides, glycoproteins, and proteins. The EPS designation (Extended Polar Selectivity) refers to a specialized bonding chemistry that provides additional polar interaction sites, offering improved selectivity for polar regions of biomolecules while maintaining controlled hydrophobic retention.
Unlike conventional endcapped C4 phases, Platinum 300 C4-EPS is not endcapped, leaving a defined degree of surface silanol activity. This enhances retention and resolution for compounds with both hydrophobic and polar characteristics, allowing fine-tuned selectivity in biopharmaceutical and proteomic applications. The large 300 Å pore size ensures excellent mass transfer and accessibility for high-molecular-weight analytes. The high-purity silica matrix provides exceptional mechanical strength, reproducibility, and stability under gradient and high-pressure conditions, making Platinum 300 C4-EPS an excellent choice for both analytical and preparative separations.
Platinum 300 C4-EPS combines optimized pore structure with extended polar selectivity – the reliable choice for precise and reproducible separations of large biomolecules in reversed-phase HPLC.
Silica-based stationary phase with C4 bonding and 300 Å pore size
Not endcapped – designed for extended polar selectivity (EPS)
Optimized for large biomolecules such as proteins and peptides
Balanced hydrophobic and polar retention for versatile separations
High-purity silica ensures mechanical stability and reproducibility
Available particle sizes: 3 µm, 5 µm, 10 µm
Separation of peptides, glycoproteins, and intact proteins
Biopharmaceutical and proteomic analysis of large biomolecules
Method development requiring complementary hydrophobic and polar selectivity
Quality control and research in biotechnology and life sciences
| Particle Sizes |
|
|---|---|
| Pore Size | 300 Å |
| Surface Area | 100 m²/g |
| Phase/Modification | C4-EPS |
| Particle Type | |
| Separation Mode | |
| USP Codes | |
| Endcapping | No |
| Phase | Material Number |
|---|---|
| Platinum 300 C4-EPS, 3 µm | 5136711 |
| Platinum 300 C4-EPS, 5 µm | 5136712 |
| Platinum 300 C4-EPS, 10 µm | 5136713 |